Shanghai Xinfan Bio: Let protein purification be as simple as plasmid extraction - Database & Sql Blog Articles

Compared to nucleic acid purification,

protein purification

But it is a very hard job. The purification time is long and the operation steps are cumbersome. Sometimes, staying in the lab for a whole day is a sad result, with low protein yield and poor purity. At this point you might think that if the purified protein is as simple as a plasmid, it would be fine.

Today, your dreams come true. Clontech(

Takara

The company has introduced a new generation of protein purification kits – His-Tagged Purification Miniprep Kit. Just like plasmid purification, loading, binding, washing, dissolving, and centrifuging, 100 μg of His-tag fusion protein can be purified in 5 minutes.

This system is at

Purification column

The nylon membrane is installed and can be operated quickly at room temperature without waiting, and is compatible with mammalian and bacterial cells. There are usually various additives in the cell lysate, such as β-mercaptoethanol, EDTA, DTT, glycerin, etc. It does not matter, this system can also stably perform high performance.

The nylon membrane in the purification column has been modified to provide a high-purity, concentrated protein with a large surface area. Through this technology, the protein binding ability of the nylon membrane is comparable to that of the resin, and even stronger. Compared with the resin, the film has a high dropping speed and can be purified in a short time. The bed is small in volume and can be eluted in small amounts and at high concentrations.

200-800 μl of cell lysate was prepared from 2-5 ml of culture medium, and after adding to the purification column, it was equilibrated, bound, washed and eluted, and purification was completed in 5 minutes, and up to 100 μg of His-tag fusion protein was obtained. The concentration is approximately 0.3-1 mg/ml. The simple and short-running process reduces the risk of protein degradation or loss of activity, making the downstream application more successful.

Elisa

Kit

, elisakit,

Shanghai

Elisa

Experimental survey

, SOD

Kit

, IgG

Kit

, IgM

Kit

, WB

Experimental generation

,

Immunohistochemistry experiment

,

Free test

,GIBCO,AMRESCO-

Shanghai Xinfan Biological